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anti wnt5a b rabbit polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti wnt5a b rabbit polyclonal antibody
    Anti Wnt5a B Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+wnt5a+b+polyclonal+antibody/WNT5A%2FB+Antibody/pm41872383-46-15-20
    Average 96 stars, based on 117 article reviews
    anti wnt5a b rabbit polyclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4°C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4°C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4◦C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4◦C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey antirabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    Blocking Assay:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4°C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4°C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4◦C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4◦C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey antirabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    Incubation:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4°C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4°C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: Following 3× PBS rinses, corneas were stained with DAPI solution (C0065, Solarbio, Beijing, China) for 5 min. Corneal flat mounts were visualized with the confocal microscope. .. For the immunostaining of CHST5 and WNT5A, corneas were fixed with Zamboni fixative for 10 min at 4◦C and washed 3 times with PBS, followed by blocking in PBS containing 0.1% Triton X-100, 2% goat serum, and 2% bovine serum albumin for 1 day and subsequently incubated with Goat Anti-Chst5 Antibody (EB09182, Absolute Biotech, Boston, USA) or Rabbit WNT5A/B Polyclonal Antibody (55184-1-AP, Proteintech Group, Rosemont, USA) overnight at 4◦C. .. After 3× PBS washes, corneas were incubated with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:400, ab150132, Abcam, Cambridge, UK) or Alexa Fluor 594-conjugated donkey antirabbit IgG (1:400, ab150076, Abcam, Cambridge, UK) diluted in 10% donkey serum for 2 h at room temperature.

    other:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies
    Article Snippet: Rabbit WNT5A/B Polyclonal Antibody , Proteintech , Cat#55184-1-AP; RRID: AB_2881285.



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